What does BSA stand for bovine?

Bovine Serum Albumin (BSA) is commonly used in cell culture protocols, particularly where protein supplementation is necessary and the other serum components are unwanted. In cell culture, it acts as a small molecule carrier.

What is bovine serum albumin used for?

The main biological function of albumin is to regulate the colloidal osmotic pressure of blood. Human and bovine albumins contain 16% nitrogen and are often used as standards in protein calibration studies. Albumin is used to solubilize lipids and is also used as a blocking agent in western blots or ELISA applications.

Why is bovine serum albumin BSA frequently used as a standard?

The bovine protein standard is the preferred standard in protein assays because in addition to its ability to increase signal in assays, bovine serum is affordable and easily mass-producible.

What is bovine serum albumin powder?

BSA is the universally accepted reference protein for total protein quantification. Powdered BSA is available in different grades- reagent, standard, protease-free, heat shock treated, cold ethanol precipitated, and fatty acid-free.

Is BSA phosphorylated?

Bovine serum albumin (BSA) was phosphorylated by the catalytic subunit of cAMP-dependent protein kinase under general protein phosphorylation conditions.

Why do we use BSA?

BSA is used because of its ability to increase signal in assays, its lack of effect in many biochemical reactions, and its low cost, since large quantities of it can be readily purified from bovine blood, a byproduct of the cattle industry.

What kind of protein is BSA?

Bovine serum albumin (BSA) is a globular protein (~66 kDa MW) that is used in numerous biochemical applications due to its stability and lack of interference with biological reactions. The BSA structure is a single polypeptide chain consisting of about 583 amino acid residues and no carbohydrates.

Where does bovine serum albumin come from?

Bovine serum albumin (BSA or “Fraction V”) is a serum albumin protein derived from cows. It is often used as a protein concentration standard in lab experiments.

Is BSA a dimer?

Analysis of these data indicated that BSA is in a monomer-dimer equilibrium with a dissociation constant of 10 +/- 2 microM at 25 degrees C in 10 mM MOPS-K (pH 5.8).

Does BSA expire?

Stability. Lyophilized BSA although stable at room temperature for 3 weeks, should be stored 2-8°C. Upon reconstitution BSA should be stored at 4°C between 2-7 days and for future use below -18°C.