What is the purpose of a concatemer?
The production of concatemers is required for regeneration of the end of the DNA molecules in viruses that possess linear genomes. The most thoroughly studied example of such a function is phage T4, in which concatemeric DNA is cleaved and then packaged into virions via a ‘headful’ mechanism.
How concatemers are formed?
In vivo [51] and in vitro [52,53] studies suggest that concatemers are formed through the annealing of the 3′-ends of the terminally redundant tails of the T7 chromosome that are generated by DNA replication. Interestingly, concatemers are not formed when E. coli RNA polymerase is not adequately inhibited.
How can concatemers be prevented?
It can be avoided by increasing the amount of SMRTbells during library prep. Currently, the RS_IsoSeq protocol detects and removes artificial concatemers.
What is concatemer DNA?
A concatemer is a long continuous DNA molecule that contains multiple copies of the same DNA sequence linked in series. These polymeric molecules are usually copies of an entire genome linked end to end and separated by cos sites (a protein binding nucleotide sequence that occurs once in each copy of the genome).
What is Catenanes in biology?
Catenanes, as the name of Latin origin suggests (catena = chain), are chain-like molecules composed of at least two cyclic compounds (i.e., a [2]catenane) that are not covalently linked to one another but nevertheless cannot be separated unless covalent bond breakage occurs.
What is a Concatemeric DNA?
What is SAGE technique?
SAGE technique works by isolating short fragments of genetic information from the expressed genes that are present in the cell under study. These unique sequence tags (9–10 base pairs in length) are concatenated serially into long DNA molecules for lump-sum sequencing [3].
What is the Headful mechanism?
A mechanism of packaging DNA in a phage head (e.g., T4) in which concatemeric DNA is cut, not at a specific position, but rather when the head is filled. This mechanism accounts for the observations of terminal redundancy and cyclic permutation in T4. From: headful mechanism in A Dictionary of Genetics »
What do you mean by terminal redundancy?
A linear DNA molecule with the same sequence (genetic information) at each end.
What is meant by Replisome?
Definition. The replisome is a large protein complex that carries out DNA replication, starting at the replication origin. It contains several enzymatic activities, such as helicase, primase and DNA polymerase and creates a replication fork to duplicate both the leading and lagging strand.
What are Rotaxanes and catenanes?
[n]Rotaxanes (Figure1A) are composed of a central dumbbell-shaped component encircled by nÀ1 trapped rings, while [n]catenanes (Figure 1B) contain n interlocked macrocycles. The prefix [n] indicates the number of me- chanically interlocked components, regardless of their identities.