What is the Jurkat cell line?
Jurkat cells are an immortalized line of T lymphocyte cells. And they are used to study acute T cell leukemia, T cell signaling, and the expression of a number of chemokine receptors susceptible to viral entry, particularly HIV. Jurkat cells are also useful in science due to their ability to produce interleukin 2.
Do Jurkat cells express CD4?
Jurkat is a CD4 T cell line provided by O. Acuto (Institut Pasteur, Paris, France). Kit 225 is a CD4 T cell line expressing high- and intermediate-affinity IL-2R (13). YT is a cell line expressing intermediate-affinity IL-2R.
Where do Jurkat cells come from?
The Jurkat cell line is an immortalized T lymphocyte cell line that was originally obtained from the peripheral blood of a boy with T cell leukemia [14].
Are Jurkat CD4 or CD8?
Jurkat is a CD4+/CD8- T-cell lymphoma that expresses an endogenous TCR. The T2 line is a TAP-deficient T-cell/B-cell hybridoma expressing HLA-A2.
Is Jurkat CD4 or CD8?
Are Jurkat cells CD4 positive?
Jurkat is a human CD4+-T-cell line that expresses CD3 along with a number of other T-cell surface receptors which are typical of freshly isolated CD4+ human peripheral blood T cells.
How do you stimulate a Jurkat cell?
Jurkat cels can be activated by exposure to a combination of anti-CD3/anti-CD28 or phytoheamaggutinin (PHA), or the C305 mAb originally developped by Arthur Weiss. IL-2 production should be measured 6 to 12 h later (whatever works best for you). 0.5 to 2 millions cells per ml works.
Do Jurkat cells express CD3?
Jurkat clones express different levels of CD3 antigen: low on 217.6 clone (A), high on 217.9 (B) and ranging from low to high levels on clone 217.7 (C).
Do Jurkat cells express CD25?
It has been found that in growing Jurkat culture within 24 h, phytohemagglutinin (PHA, 5 μg/ml) or PHA in combination with 12,13-phorbol dibutirate (PDBu, 10(-8)M) increase the number of CD25+ cells to 32.3 ± 3.4% (n = 11) and 44.8 ± 8.6% (n = 6) respectively.
What is the best way to transfect Jurkat cells?
In our lab we work with Jurkat cells and the best way to transfect them is electroporation (if you don´t use lentivirus) and works very well for IPs, luciferase assays, CD3-activation experiments. If you need the cells for microscopy experiments or to get high levels of transfection, we use sometimes Neon system but it is more expensive
What is the transfection efficiency of jurket cells with Neon Transfection?
We tested the transfection efficiency in Jurket cells with Neon Transfection System and the transfection efficiency can achieve 94%. Please see attached file for the parameters, efficiency and cell viability.
What is the best reagent for siRNA transfection of Jurkat cells?
for siRNA transfection of JURKAT cells my colleagues use Viromer BLUE (from Lipocalyx). By using Viromers they get up to 70% transfection efficiency. Viromers are synthetic polymers which are zero in charge. Due to their endosome escape mechanism transfection efficiency is higher compared to standard reagents.
What is the best method for lentiviral transduction for Jurkat?
For Jurkat there is a special method for lentiviral transduction called spinoculation. I simplified this method by just spinning the cells with the virus in 15ml tubes 30 min 800g 32C in swing bucket rotor. Afterr that suspended and plated without changing the medium.