What is STS technique?
Sequence-tagged site (STS) mapping This technique maps the positions of short DNA sequences (between 200-500 base pairs in length) that are easily recognisable and only occur once in the genome. These short DNA sequences are called sequence-tagged sites (STSs).
What is STS in genome?
Sequence-Tagged Site (STS) is a relatively short, easily PCR-amplified sequence (200 to 500 bp) which can be specifically amplified by PCR and detected in the presence of all other genomic sequences and whose location in the genome is mapped. The STS concept was introduced by Olson et al (1989).
What is STS content mapping?
The basic method used, called ‘STS-content mapping,’ involves the use of sequence-tagged sites (STSs) as markers and YACs as the source of cloned DNA. An STS is a short segment of genomic DNA that can be uniquely detected using a PCR assay.
What are the types of STS?
Three classes of STS loci are recognized: type I, type II, and type III (O’Brien et al., 1999). Type I loci are based on single- copy coding genes (exons and introns) and help define homologous landmarks that are conserved across a wide range of taxa.
What is simple tagged sequence?
From Wikipedia, the free encyclopedia. A sequence-tagged site (or STS) is a short (200 to 500 base pair) DNA sequence that has a single occurrence in the genome and whose location and base sequence are known.
What are scar markers?
A “SCAR”(Sequence Characterized Amplified Region), initially developed for downy mildew resistance genes in lettuce [4], is PCR-based monolocus codominant marker that requires the use of two specific primers designed from nucleotide sequence established in cloned RAPD fragment linked to a trait of interest(Fig. 1).
What is clone by clone sequencing?
During clone-by-clone sequencing, a map of each chromosome of the genome is made before the DNA is split up into fragments ready for sequencing.
What are CAPS markers?
Cleaved amplified polymorphic sequence (CAPS) marker is the combination of PCR and RFLP techniques where a very small amount of DNA is required for PCR analysis to show polymorphisms. CAPS markers were successfully employed in many perennial plants due to the availability of their nucleotide sequence in public domain.
How useful ESTs are?
ESTs have proven enormously useful in delineating the gene content of organisms and the expression patterns of genes in various cells and tissues. Several hundred thousand EST sequences would reveal most of the abundant messages for an organism and many of the rare messages as well.
What is the Blast program?
BLAST is an acronym for Basic Local Alignment Search Tool and refers to a suite of programs used to generate alignments between a nucleotide or protein sequence, referred to as a “query” and nucleotide or protein sequences within a database, referred to as “subject” sequences.
What is STS (sequence-tagged site)?
Sequence-Tagged Site (STS) is a relatively short, easily PCR-amplified sequence (200 to 500 bp) which can be specifically amplified by PCR and detected in the presence of all other genomic sequences and whose location in the genome is mapped. The STS concept was introduced by Olson et al (1989).
What does STS stand for in biology?
Sequence-tagged site From Wikipedia, the free encyclopedia A sequence-tagged site (or STS) is a short (200 to 500 base pair) DNA sequence that has a single occurrence in the genome and whose location and base sequence are known.
What is Sts in genome sequencing?
Sequence-Tagged Site (STS) is a relatively short, easily PCR-amplified sequence (200 to 500 bp) which can be specifically amplified by PCR and detected in the presence of all other genomic sequences and whose location in the genome is mapped.
Who is STS?
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