What is the microplate spectrophotometer used for?

The microplate is designed to measure absorbance in small volumes. It is typically used to determine the concentration of nucleic acids and proteins. It contains 16 spots which can be loaded with 2 microliters of different samples or replicates for subsequent absorbance measurements.

How much does a microplate reader cost?

BioTek’s simplest ELISA reader, for instance, costs about $5,000, says Xavier Amouretti, product marketing manager at BioTek Instruments, a company that sells a range of microplate reader systems; the company’s least-expensive multimode reader, the Synergy™ HT, costs about $20,000.

What is the microplate reader spectrophotometer?

Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader.

How does a microplate reader measure absorbance?

The light detector is located on the opposite side of the microplate relative to the light source. It measures how much of the total light intensity is transmitted through the sample and at which wavelengths. Light that does not pass through to the detector is absorbed by the sample.

What is multi mode plate reader?

Multi-mode microplate readers are used in different research fields for the quantification of different biological and chemical assays in a microplate. These assays span over a wide wavelength range, generally from 220-900 nm in different detection modes. They are very complex instruments, optically speaking.

What would be the advantage of using a microplate reader versus a single cuvette reading of samples?

Compared to a standard spectrophotometer that measures one sample at a time in a cuvette, an absorbance microplate reader can measure the same application with higher throughput. It can measure a full 96 well microplate in seconds, or 384 or 1536 well plates in a few minutes.

How does ELISA reader work?

What an ELISA Reader Does. An ELISA reader measures and quantitates the color differences in the 12 wells of the plate. ELISA readers or micro plate readers do spectrophotometry; they emit light at one wave length, and measure the amount of light absorbed and reflected by an object such as a protein.

Can the spectramax 340pc microplate reader run standard spectrophotometer applications?

The Molecular Devices SpectraMax 340PC Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument.

What types of absorbance assays does the spectramax 340pc384 support?

The SpectraMax 340PC384 Microplate reader supports various absorbance assay categories, such as protein quantitation, endotoxin detection, microbial growth, ELISAs and immunoassays, reporter gene assays, phosphotases/kinases, enzyme assays, and cell viability, proliferation, and cytotoxicity.

What is the spectramax® i3x microplate reader?

A streamlined workflow for cell counting and phenotypic characterization is critical to many experiments. Our SpectraMax® i3x microplate reader with MiniMax™ cytometer provides you w… Ionic Transport Assays Inc. is a contract research organization located in St Louis, MO.

What is the spectramax® ABS microplate reader?

The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems.The SpectraMax® ABS Plus Mi… Nucleic acid and protein quantitation are essential measurements upstream of many sophisticated assays in genetics and molecular biology.