What is lambda repressor?
The lambda repressor is a self assembling dimer also known as the cI protein. It binds DNA in the helix-turn-helix binding motif. It regulates the transcription of the cI protein and the Cro protein. The life cycle of lambda phages is controlled by cI and Cro proteins.
What are transducing particles?
A defective phage carrying part of the Host genome in place of part of the phage genome.
What is lambda DNA used for?
Lambda DNA (48,502 bp) may be used as a molecular weight size marker during nucleic acid gel analysis following digestion with a restriction enzyme (such as HindIII). Lambda DNA can also be used as a substrate in restriction enzyme activity assays.
What does lambda mean in biology?
Enterobacteria phage λ (lambda phage, coliphage λ) is a bacterial virus that infects the bacterial species Escherichia coli.
What is Lysogeny in microbiology?
lysogeny, type of life cycle that takes place when a bacteriophage infects certain types of bacteria. In this process, the genome (the collection of genes in the nucleic acid core of a virus) of the bacteriophage stably integrates into the chromosome of the host bacterium and replicates in concert with it.
How does lambda repressor and Cro work?
Cro, a repressor protein of temperate bacteriophages (e.g. lambda [l], 434, P22), works in opposition to the phage’s repressor to control the genetic switch that determines whether a lytic or lysogenic cycle will follow infection.
What are transducing phages?
Transducing particles are ostensibly like mature phage particles, only they eject bacterial DNA instead of a viral genome when they infect other cells. The DNA can then recombine into the chromosome or replicate as a plasmid in the new host cell.
Which kind of transduction occurs by bacteriophage lambda?
Due to the location of lambda phage in E. coli genome, all the genes are transduced. So, the trp, gal, bio genes are tranduced due to site-specific recombination in specialized transduction. Thus, the correct answer is option D.
How many ecor1 sites are there in Lambda DNA?
The DNA from bacteriophage lambda is a well-characterized linear molecule containing six recognition sites for Eco RI (generating 5 fragments with distinct sizes and 2 fragments that are very close in size). In this experiment, Lambda DNA is digested by the Eco RI endonuclease.
What is a lambda in physics?
Wavelength is usually denoted by the Greek letter lambda (λ); it is equal to the speed (v) of a wave train in a medium divided by its frequency (f): λ = v/f. wavelength.
What does lambda mean in chemistry?
Lambda (written λ, in lowercase) is a non-SI unit of volume equal to 10−9 m3, 1 cubic millimetre (mm3) or 1 microlitre (μL). Introduced by the BIPM in 1880, the lambda has been used in chemistry and in law for measuring volume, but its use is not recommended.
What percentage of wild type lambda are released from E coli?
A strain of E. coliis simultaneously infected with lambda galand lambda biotransducing phages at a multiplicity of infection (moi) of approximately 5 for each phage. After the culture lyses, approximately 10% of the phage released are wild-type lambda.
How many different Lambda dgalphage are there?
Four different lambda dgalphage were isolated. These phage were then crossed against four lambda point mutants, selecting for wild-type recombinants. The lambda dgalphage were also crossed against four different E. coli galmutants, selecting for Gal+recombinants.
Is it possible to obtain specialized transducing derivatives of P22?
Although P22 is a generalized transducing phage, it is also possible to obtain specialized transducing derivatives of P22. For example, specialized transducing particles have been obtained for the newDand supQgenes which are located adjacent to the P22 attachment site on the S. typhimurium chromosome (ataA).