What is isolectin IB4?
Isolectin-IB4 is a 114 kDa protein isolated from the seeds of the African legume, Griffonia simplicifolia and as such is known to bind to microglia, group B erythrocytes, perivascular cells, and endothelial cells (Grossmann et al., 2002, Tarpley et al., 2004).
What is IB4?
Note: An IB4 is used by the Paying State to request a transfer of wages for use in a Combined Wage Claim when someone has worked in two or more states.
What is isolectin?
Isolectin GS-IB4 is a 114,000-dalton glycoprotein that is part of a family of five tetrameric type I isolectins (IA4, IA3B, IA2B2, IAB3, and IB4) isolated from the seeds of the tropical African legume Griffonia simplicifolia.
What does Isolectin bind B4?
Introduction. Isolectin-B4 (IB4) from the plant Griffonia simplicifolia binds to small dorsal root ganglion (DRG) neurons through α-d-galactose carbohydrate residues on their soma membranes (Silverman and Kruger, 1990; Fullmer et al., 2004).
What does IB4 label?
IB4 is a lectin from the plant Griffonia simplicifolia that binds to α-d-galactose residues in glycoconjugates on the cell surface and Golgi apparatus of small, NF-poor DRG neurons in a variety of species. 214.
What is IB4 a marker for?
IB4-positive neurons express receptors for glial cell line-derived neurotrophic factor (GDNF) and depend on GDNF for survival after birth (Bennett et al. 1998). IB4 positive neurons selectively express the P2X3 Receptor for ATP.
What is Isolectin IB4?
Description: Isolectin IB4 is a 114 kDa glycoprotein and part of a family of five tetrameric type I isolectins (IA4, IA3B, IA2B2, IAB3, and IB4) isolated from the seeds of the tropical African legume Griffonia simplicifolia (formerly Bandeiraea simplicifolia).
What are the limitations of lectins?
One common caveat for all lectins is their cross-reactivity with endothelial cells. While blood vessels are rarely mistaken for microglial cells, abundant staining of endothelial cells can hamper the identification of the smaller microglia.
What does IB4 bind to in microglia?
The isolectin IB4 binds RET receptor tyrosine kinase in microglia Ret receptor tyrosine kinase is the signaling component of the receptor complex for the family ligands of the glial cell line-derived neurotrophic factor (GDNF).
What are the uses of Alexa Fluor® 647 Isolectin gs-ib4 conjugate?
The far-red fluorescent Alexa Fluor® 647 isolectin GS-IB4 conjugate can be used in fluorescence microscopy or flow cytometry. For Research Use Only.