What is the brightest Fluorochrome?
PE (Ex-Max 496 nm/Em-Max 578 nm): R-phycoerythrin – is a pigment naturally found in red algae where it transfers light energy to chlorophyll during photosynthesis. PE has one of the largest absorption co-efficiencies making it one of the brightest fluorochromes available to date.
What is the brightest fluorophore?
It is concluded that cyanine 3.18 should be useful for multicolor fluorescence experiments and that it may be the brightest fluorophore available for single-color fluorescence immunocytochemistry.
What is brightness index?
The Fluorophore Brightness Index Score is a relative indication of fluorescence intensity above the background for each fluorophore antibody conjugation (1=dim, 5=brightest).
Does PE overlap APC?
Furthermore, PE (and PE-Texas Red to an even greater extent) does overlap significantly with APC.
Is APC far red?
Allophycocyanin (APC) is an intensely bright phycobiliprotein isolated from red algae that exhibits far-red fluorescence with high quantum yields. It is excited by laser lines at 594 and 633 nm, with an absorbance maximum at 650 nm and a fluorescence emission peak at 660 nm.
What is eFluor™ 450?
Cell Proliferation Dye eFluor™ 450 is a violet fluorescent dye that can be used to monitor individual cell divisions.
How does formaldehyde affect the fluorescence of eFluor 450?
Stability data shows eFluor 450 exhibits minimal loss of fluorescence, when cells are exposed to a formaldehyde fixative treatment for up to 24 hours. eFluor 450 dye is a small-molecule fluorescent dye with spectral properties similar to Pacific Blue dye.
What is cell proliferation dye eFluor™ 450?
Cell Proliferation Dye eFluor™ 450 has a peak excitation of 409 nm and can be detected using a 450/50 band pass filter (equivalent to eFluor™ 450 or Pacific Blue™), making it compatible with applications that utilize GFP. Cell Proliferation Dye eFluor™ 450 is supplied as a lyophilized powder.