What is secreted alkaline phosphatase assay?
The SEAP Reporter Gene Assay is designed specifically for measuring secreted placental AP (alkaline phosphatase) in conditioned cell culture medium from transfected cells. SEAP is secreted into the culture supernatant where it can be easily sampled and assayed.
What is secreted embryonic alkaline phosphatase?
Secreted embryonic alkaline phosphatase (SEAP) is a reporter widely used to study promoter activity or gene expression. It is a truncated form of human placental alkaline phosphatase (PLAP) by deletion of the GPI anchor.
What is a reporter in biology?
In molecular biology, a reporter gene (often simply reporter) is a gene that researchers attach to a regulatory sequence of another gene of interest in bacteria, cell culture, animals or plants.
What is HEK blue?
HEK-Blue™ Detection is a cell culture medium that allows the detection of SEAP (Secreted embryonic alkaline phosphatase) as the reporter protein is secreted by the cells. HEK-Blue™ Detection contains all the nutrients necessary for cell growth and a specific SEAP color substrate.
What is quanti blue?
QUANTI-Blue™ Solution is a colorimetric enzyme assay developed to determine any alkaline phosphatase (AP) activity in a biological sample, such as cell culture supernatant. It has been optimized for use in 96-well plates (standard procedure) and in 1536-well plates (high-throughput screening procedure).
Is firefly luciferase secreted?
However, Cypridina and Gaussia luciferases are secreted; therefore, Pierce Cypridina-Firefly and Gaussia-Firefly dual-spectral luciferase kits are compatible with time-course assays.
What is reporter or marker gene?
There are two types of marker genes: a selectable marker (Antibiotics ) and a marker for screening(Green fluorescent protein ). a reporter gene (often simply reporter) is a gene that researchers attach to a regulatory sequence of another gene of interest in cell culture, animals or plants.
How does quanti blue work?
QUANTI-Blue™ is a colorimetric enzyme assay developed to determine any alkaline phosphatase activity (AP) in a biological sample, such as supernatants of cell cultures. QUANTI-Blue™ Solution changes from pink to a purple-blue color in the presence of AP.