What is Lambda HindIII DNA marker and what is its function?
Thermo Scientific Lambda DNA/HindIII Marker is recommended for sizing of linear double-stranded large DNA fragments in agarose gels. Lambda DNA is digested to completion with the appropriate Thermo Scientific restriction enzyme(s) and purified and dissolved in storage buffer.
What is HindIII Lambda Digest?
The HindIII digest of lambda DNA (cI857ind1 Sam 7) yields 8 fragments suitable for use as molecular weight standards for agarose gel electrophoresis (1). The approximate mass of DNA in each of the bands is provided (assuming a 1.0 μg load) for approximating the mass of DNA in comparably intense samples of similar size.
Is lambda DNA double-stranded?
Lambda DNA, commonly used as a DNA marker, control, or substrate, is available from several sources. This linear, double-stranded DNA strand of 48,502 bp originates from the E. coli bacteriophage lambda.
What is a diagnostic digest?
The goal of a diagnostic digest is to cut your plasmid into specific sized pieces and analyze the resulting fragments by gel electrophoresis. The pattern of the fragments on the gel can indicate if the plasmid contains the expected size insert.
What is the size of the lambda genome in kilobases?
The DNA of Bacteriophage λ is approximately 48,514 base pairs or 48.514 kilobase pairs in length while the human genome is approximately 3 billion base pairs.
How many DNA fragments are produced by HaeIII?
11 fragments
Thus treatment of this DNA with the enzyme produces 11 fragments, each with a precise length and nucleotide sequence. These fragments can be separated from one another and the sequence of each determined. HaeIII and AluI cut straight across the double helix producing “blunt” ends.
How is the lambda DNA marker digested and purified?
Lambda DNA is digested to completion with the appropriate Thermo Scientific restriction enzyme (s) and purified and dissolved in storage buffer. DNA fragments contain various sticky ends depending on the restriction enzyme used for preparation of the marker. The marker can be labeled radioactively with T4 Polynucleotide Kinase.
What is Thermo Scientific lambda DNA marker?
Description Thermo Scientific Lambda DNA/HindIII Marker is recommended for sizing of linear double-stranded large DNA fragments in agarose gels. Lambda DNA is digested to completion with the appropriate Thermo Scientific restriction enzyme (s) and purified and dissolved in storage buffer.
How many DNA fragments does HindIII digest of lambda DNA produce?
The HindIII digest of lambda DNA (cI857ind1 Sam 7) yields 8 fragments suitable for use as molecular weight standards for agarose gel electrophoresis (1). The approximate mass of DNA in each of the bands is provided (assuming a 1.0 μg load) for approximating the mass of DNA in comparably intense samples of similar size.
How do you separate the cohesive ends of lambda DNA fragments?
The cohesive ends (the 12 nt cos site of lambda DNA) of fragments indicated may anneal and form additional bands. These fragments can be separated by heating at 65°C for 5 minutes and then cooling on ice for 3 minutes. For Research Use Only. Not for use in diagnostic procedures. Convenient, on-site access to the products you need. Learn more.